(3/14) NIG and DDBJ network services temporary down
.(Feb.18, 2010)
(2/16) Homology Search and ClustalW temporary down
.(Feb.10, 2010)
(3/31) Termination of a part of DDBJ services
.(Feb.01, 2010)
PROGRAM
:
blastn
(DNA query vs. DNA database)
blastx
(DNA query[translated into protein] vs. Protein database)
tblastx
(DNA query[translated into protein] vs. DNA database[translated into protein])
blastp
(Protein query vs. Protein database)
tblastn
(Protein query vs. DNA database[translated into protein])
QUERY SEQUENCE NAME, QUERY SEQUENCE
:
* The name of a sequence can be attached at first line with ">" at line head. You can use "File Upload" or fill the box directly.
* Multiple query sequence possible.
Example
* The query sequence is
filtered
for low complexity regions by default.
File Upload:
or COPY & PASTE:
RESULT
:
WWW
Graphical View (<= 100 sequences)
* Alignments can be seen graphically when "Graphical View" is checked.
E-Mail
HTML format
DATABASE
:
Available databases vary according to the programs chosen.
DNA DATABASE
DDBJ ALL (DDBJ periodical release + daily updates)
DDBJ updates
EPD (Eukaryotic Promoter Database)
16S rRNA (Prokaryotes)
DIVISION
:
Effective only when "DDBJ ALL" or "DDBJ updates" is selected.
Checked divisions will be searched.
default
select-all
clear-all
Human
Primates
Rodents
Mammals
Vertebrates
Invertebrates
Plants
Bacteria
Viruses
Phages
Unannotated Seq
GSS
HTG
Patent
STS
Synthetic DNAs
HTC
ENV
TSA
EST division
select-all
clear-all
A.thaliana
B.taurus
C.elegans
C.intestinalis
C.reinhardtii
D.discoideum
D.melanogaster
D.rerio
G.gallus
G.max
H.sapiens
H.vulgare
M.musculus
M.truncatula
O.sativa
R.norvegicus
S.lycopersicum
T.aestivum
X.laevis
X.tropicalis
Z.mays
Others
PROTEIN DATABASE
Protein default data(UniProt + PRF + PDB)
UniProt (UniProt/Swiss-Prot + UniProt/TrEMBL)
UniProt/Swiss-Prot
UniProt/TrEMBL
DAD
PRF
PDB
C.elegans[wormpep]
Gap
:
ON
OFF
detailed options
SCORES
:
ALIGNMENTS
:
EXPECT
:
SCORING MATRIX
(ineffective when blastn selected) :
default
PAM30
PAM70
PAM250
BLOSUM45
BLOSUM50
BLOSUM62
BLOSUM80
BLOSUM90
FILTER
:
ON
OFF
WORD SIZE
:
OTHER OPTIONS
:
Please refer to
manual
of BLAST for a detailed explanation of these options and parameters.