(3/14) NIG and DDBJ network services temporary down.(Feb.18, 2010)
(2/16) Homology Search and ClustalW temporary down.(Feb.10, 2010)
(3/31) Termination of a part of DDBJ services.(Feb.01, 2010)

  • PROGRAM :
    blastn (DNA query vs. DNA database)
    blastx (DNA query[translated into protein] vs. Protein database)
    tblastx (DNA query[translated into protein] vs. DNA database[translated into protein])
    blastp (Protein query vs. Protein database)
    tblastn (Protein query vs. DNA database[translated into protein])
  • QUERY SEQUENCE NAME, QUERY SEQUENCE :
    * The name of a sequence can be attached at first line with ">" at line head. You can use "File Upload" or fill the box directly.
    * Multiple query sequence possible. Example
    * The query sequence is filtered for low complexity regions by default.
    File Upload:
    or COPY & PASTE:
  • RESULT :
    WWW Graphical View (<= 100 sequences)
    * Alignments can be seen graphically when "Graphical View" is checked.
    E-Mail HTML format
  • DATABASE :
    Available databases vary according to the programs chosen.
  • DNA DATABASE
  • DDBJ ALL (DDBJ periodical release + daily updates)
    DDBJ updates
    EPD (Eukaryotic Promoter Database)    
    16S rRNA (Prokaryotes)
       
  • DIVISION : Effective only when "DDBJ ALL" or "DDBJ updates" is selected.
  • Checked divisions will be searched. default select-all clear-all
    Human Primates Rodents Mammals Vertebrates Invertebrates
    Plants Bacteria Viruses Phages Unannotated Seq GSS
    HTG Patent STS Synthetic DNAs HTC ENV
    TSA
    EST division select-all clear-all
    A.thaliana B.taurus C.elegans C.intestinalis C.reinhardtii
    D.discoideum D.melanogaster D.rerio G.gallus G.max
    H.sapiens H.vulgare M.musculus M.truncatula O.sativa
    R.norvegicus S.lycopersicum T.aestivum X.laevis X.tropicalis
    Z.mays Others
  • PROTEIN DATABASE
  • Protein default data(UniProt + PRF + PDB)
    UniProt (UniProt/Swiss-Prot + UniProt/TrEMBL)
    UniProt/Swiss-Prot UniProt/TrEMBL DAD
    PRF PDB C.elegans[wormpep]
  • Gap :
    ON OFF

  • detailed options

  • SCORES :
  • ALIGNMENTS :
  • EXPECT :
  • SCORING MATRIX (ineffective when blastn selected) :
  • FILTER :
    ON OFF
  • WORD SIZE :
  • OTHER OPTIONS :

  • Please refer to manual of BLAST for a detailed explanation of these options and parameters.